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Pubblicazioni - Colombo Dott. Jacopo
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Detection and quantification of SARS-CoV-2 by droplet digital PCR in real-time PCR negative nasopharyngeal swabs from suspected COVID-19 patients.
PLoS One2020 ;15(9):e0236311. doi: 10.1371/journal.pone.0236311.
Alteri Claudia, Cento Valeria, Antonello Maria, Colagrossi Luna, Merli Marco, Ughi Nicola, Renica Silvia, Matarazzo Elisa, Di Ruscio Federica, Tartaglione Livia, Colombo Jacopo, Grimaldi Chiara, Carta Stefania, Nava Alice, Costabile Valentino, Baiguera Chiara, Campisi Daniela, Fanti Diana, Vismara Chiara, Fumagalli Roberto, Scaglione Francesco, Epis Oscar Massimiliano, Puoti Massimo, Perno Carlo Federico
Abstract
Since SARS-CoV-2-based disease (COVID-19) spreads as a pandemic, the necessity of a highly sensitive molecular diagnosis that can drastically reduce false negatives reverse transcription PCR (rtPCR) results, raises as a major clinical need. Here we evaluated the performance of a ddPCR-based assay to quantify SARS-CoV-2 titer in 55 suspected COVID-19 cases with negative rtPCR results thanks to in-house ddPCR assay (targeting RdRp and host RNaseP). Samples were collected at ASST-GOM Niguarda between February and May 2020 at hospital admission. Clinical and imaging data were obtained for clinical staging and definition of disease severity. Patients were mainly female (45.5%) with a median age of 73 (57-84) years. ddPCR-based assay detected SARS-CoV-2 genome in nasopharyngeal samples of 19 (34.5%) patients (median viral-load: 128 copies/mL, IQR: 72-345). In 15 of them (78.9%), chest CT showed a classical COVID-19 bilateral interstitial pneumonia; 14 patients (73.7%) showed severe COVID-19 manifestations. ddPCR did not identify any trace of SARS-CoV-2 genome in the respiratory samples of the remaining 36 patients. The serological assay performed in a subgroup of 34 patients at the later stage of illness (from 3 days to 90 days after) confirmed the presence of SARS-CoV-2 antibodies in all patients tested positive for SARS-CoV-2 in ddPCR (100%). Contrariwise, negative tests were observed in 95.0% ddPCR negative patients (P<0.001). Thanks to a ddPCR-based assay, we achieved a rapid and accurate SARS-CoV-2 diagnosis in rtPCR-negative respiratory samples of individuals with COVID-19 suspect, allowing the rapid taking care and correct management of these patients.
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Quantification of 1,3-?-d-glucan by Wako ?-glucan assay for rapid exclusion of invasive fungal infections in critical patients: A diagnostic test accuracy study.
Mycoses2020 Aug;():. doi: 10.1111/myc.13170.
Cento Valeria, Alteri Claudia, Mancini Valentina, Gatti Milo, Lepera Valentina, Mazza Ernestina, Moioli Maria Cristina, Merli Marco, Colombo Jacopo, Orcese Carlo Andrea, Bielli Alessandra, Torri Stefania, Gasparini Laura Elisa, Vismara Chiara, De Gasperi Andrea, Brioschi Paolo, Puoti Massimo, Cairoli Roberto, Lombardi Gianluigi, Perno Carlo Federico
Abstract
OBJECTIVES:
Rapid and reliable exclusion of invasive fungal infections (IFI) by markers able to avoid unnecessary empirical antifungal treatment is still a critical unmet clinical need. We investigated the diagnostic performance of a newly available ?-d-Glucan (BDG) quantification assay, focusing on the optimisation of the BDG cut-off values for IFI exclusion.
METHODS:
BDG results by Wako ?-glucan assay (lower limit of detection [LLOD] = 2.16 pg/mL, positivity ? 11 pg/mL) on two consecutive serum samples were retrospectively analysed in 170 patients, admitted to haematological wards (N = 42), intensive care units (ICUs; N = 80), or other wards (N = 48), exhibiting clinical signs and/or symptoms suspected for IFI. Only patients with proven IFI (EORTC/MSG criteria) were considered as true positives in the assessment of BDG sensitivity, specificity and predictive values.
RESULTS:
Patients were diagnosed with no IFI (69.4%), proven IFI (25.3%) or probable IFI (5.3%). Two consecutive BDG values < LLOD performed within a median of 1 (interquartile range: 1-3) day were able to exclude a proven IFI with 100% sensitivity and negative predictive value (primary study goal). Test's specificity improved by using two distinct positivity and negativity cut-offs (7.7 pg/mL and LLOD, respectively), but remained suboptimal in ICU patients (50%), as compared to haematological or other patients (93% and 90%, respectively).
CONCLUSIONS:
The classification of Wako's results as negative when < LLOD, and positive when > 7.7 pg/mL, could be a promising diagnostic approach to confidently rule out an IFI in both ICU and non-ICU patients. The poor specificity in the ICU setting remains a concern, due to the difficulty to interpret positive results in this fragile population.
© 2020 Wiley-VCH GmbH.
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Detection of strong inspiratory efforts from the analysis of central venous pressure swings: a preliminary clinical study.
Minerva Anestesiol2020 Aug;():. doi: 10.23736/S0375-9393.20.14323-2.
Colombo Jacopo, Spinelli Elena, Grasselli Giacomo, Pesenti Antonio M, Protti Alessandro
Abstract
BACKGROUND:
Swings of central venous pressure (?CVP) may reflect those of pleural and esophageal (?PES) pressure and, therefore, the strength of inspiration. Strong inspiratory efforts can produce some harm. Herein we preliminarily assessed the diagnostic accuracy of ?CVP for strong inspiratory efforts in critically-ill subjects breathing spontaneously.
METHODS:
We measured ?CVP and ?PES in 48 critically-ill subjects breathing spontaneously with zero end-expiratory pressure (ZEEP) or 10 cmH2O of continuous positive airway pressure (CPAP). The overall diagnostic accuracy of ?CVP for strong inspiratory efforts (arbitrarily defined as ?PES >8 mmHg) was described as the area under the receiver operating characteristic (ROC) curve, with 0.50 indicating random guess. The agreement between ?CVP and ?PES was assessed with the Bland-Altman analysis.
RESULTS:
?CVP recognized strong inspiratory efforts with an area under the ROC curve of 0.95 (95% confidence intervals, 0.85-0.99) with ZEEP and 0.89 (0.76-0.96) with CPAP, both significantly larger than 0.50 (p<0.001). With the best cut-off value around 8 mmHg, the diagnostic accuracy of ?CVP was 0.92 (0.80-0.98) with ZEEP and 0.94 (0.83-0.99) with CPAP. With ZEEP, the median difference between ?CVP and ?PES (bias) was -0.2 mmHg, and the 95% limits of agreement (LoA) were -3.9 and +5.5 mmHg. With CPAP, bias was -0.1 mmHg, and 95%-LoA were -5.8 and +4.5 mmHg. In both cases, ?CVP correlated with ?PES (rs 0.81 and 0.67; p<0.001 for both).
CONCLUSIONS:
In critically-ill subjects breathing spontaneously, ?CVP recognized strong inspiratory efforts with acceptable accuracy. Even so, it sometimes largely differed from ?PES.
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[Platypnea-orthodeoxia syndrome associated with patent foramen ovale and aortic root aneurysm].
G Ital Cardiol (Rome)2020 Jul;21(7):562-563. doi: 10.1714/3386.33646.
Bottiroli Maurizio, Vignati Gabriele, Cannata Aldo, Colombo Jacopo, Pinciroli Riccardo, Mondino Michele
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Risk factors for intubation in severe bronchiolitis: a useful tool to decide on an early intensive respiratory support.
Minerva Pediatr2020 06;():. doi: 10.23736/S0026-4946.20.05574-7.
Colombo Jacopo, Gattoni Chiara, Carobbio Alessandra, Nacoti Mirco, Pellicioli Isabella, Vedovati Sergio, Bonanomi Ezio
Abstract
BACKGROUND:
Bronchiolitis is the most frequent lower airway infection leading hospitalization in children younger than 2 years. RSV is the typical common cause, followed by rhinovirus. Criteria for Pediatric Intensive Care Unit (PICU) admission are not defined by guidelines.
METHODS:
A retrospective analysis of children with severe bronchiolitis admitted from 2013 to 2016 to our PICU was performed to to identify the risk factors associated with intubation in this population. Fourteen variables were studied: sex, weight, age, nationality, provenience, duration of symptoms, risk factors for bronchiolitis development, recurrence, apnea, SpO2 in air, Modified Wood's Clinical Asthma score (M-WCAS), microbiological results, medical treatment, CPAP therapy. The relationship between these variables and the need for mechanical ventilation were explored using univariate and multivariate logistic regression analysis. A ROC analysis was used to identify cut-off for the continuous variables identified as risk factors for intubation in multivariate analysis.
RESULTS:
We enrolled 93 patients, 19 (20.4%) were intubated. Univariate and multivariate analysis demonstrated that a M-WCAS score ? 7, SpO2 ? 75% and apnea were significantly associated to intubation in children with severe bronchiolitis.
CONCLUSIONS:
Cut-off values of the variables identified as risk factors for intubation may represent an important tool for pediatricians to decide a prompt and appropriate intensive respiratory support.
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Clinical osseointegration of bone level implants with conical shape and textured surface with low primary stability.
Minerva Stomatol2020 Feb;69(1):8-13. doi: 10.23736/S0026-4970.20.04277-6.
Baldi Domenico, Colombo Jacopo, Verardi Simone, Rebaudi Alberto, Rebaudi Federico, Makary Christian
Abstract
BACKGROUND:
Evidence shows lower chance for osseointegration of implants without sufficient primary stability. The present retrospective study observed bone level conical screw implants with textured surface without primary stability.
METHODS:
Twenty-six Stark conical screw implants, with V-Blast (Vanish Blast) surface treatment were placed with low primary stability, (insertion torque lower than 10 N/cm and visible mobility at lateral load of 250 g). A soft diet was prescribed. osseointegration was assessed applying 30 Ncm of reverse torque 6 months after placement.
RESULTS:
The 26 implants that did not achieve primary stability still had a survival rate of 96% after the observation period and were classified as successful according with ICOI Pisa consensus conference success, survival, failure classification .96% of the implants showed clinical osteointegration and were successfully restored. After 12 months, all implants remained functional.
CONCLUSIONS:
It can be concluded that bone-level implants with V-Blast surface in absence of functional loading are able to achieve osteointegration, even with low primary stability.
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Pulp vitality during ultrasonic tooth preparation. Part 2.
Minerva Stomatol2020 Feb;69(1):21-26. doi: 10.23736/S0026-4970.19.04280-8.
Baldi Domenico, Colombo Jacopo, Stacchi Claudio, Menini Maria, Oronos Amalia, Pera Paolo
Abstract
BACKGROUND:
Ultrasonic devices have a lot of dental application specially in hygiene and surgery. However there are some disadvantages like cut slowness and heat production. Prosthodontics application is less investigated. In particular the heat production could be an important factor for the clinician. In fact more than 5.5 °C temperature increasing could determine pulp necrosis during tooth preparation. The aim of this study is to investigate the increasing of temperature during finishing line repositioning and polishing phases using ultrasounds inserts and to verify if it remains within the limit of 5.5 °C.
METHODS:
A sample of 32 human molars (extracted for periodontal reasons) was selected. The teeth were endodontically treated and prepared with burs in order to obtain a prosthetic round chamfer preparation, leaving the apical portion pervious for inserting the thermocouple probe. Then, they were inserted in plaster cubes up to the cement-enamel junction. A wall has been selected for each tooth for margin repositioning and finishing and prepared with the piezoelectric instrument (Multipiezo Touch with TipHolder DB2, Mectron, Carasco, Genoa, Italy). A mechanical arm was used to standardize the operator-dependent parameters. These parameters were: the pressure exerted on the dental wall, the cutting length and the time required for margin repositioning and finishing. The test phase consisted in a first stage of margin repositioning using an regular ultrasound tip with a diamond grain of 120 micron (DB120, Mectron, Carasco, Italy) (group 1), followed by a second finishing step conducted by a extra fine ultrasound tip with a diamond grain of 60 micron (DB60, Mectron, Carasco, Italy) (group 2). Each test lasted 60 seconds: this was the time that the mechanical arm needed to accomplish 32 rides. During these stages the intrapulpal temperature has been recorded thanks to a thermocouple. The obtained temperature data were analyzed by Kruskal-Wallis test and Mann-Withney post-hoc test, without Bonferroni correction (P<0.001).
RESULTS:
The average pulpal temperature increase was 4.65 °C with a standard deviation of 0.99 °C for the DB 120 ultrasonic tip and 5.40 °C with a standard deviation of 0.84 °C for the DB 60 ultrasonic tip. However, neither of the instruments reach the medium critical level of 5.5°C reported in the literature, there are some single values who exceed it. There is statistically significant difference using tips with different granulometries within the two groups (P value =0.013).
CONCLUSIONS:
Ultrasonic tools are very performing to achieve results in repositioning and polishing of prepared tooth. The in-vitro analysis show that the pulp temperature increasing remains within the safe limits literature shows. It is important underline the polishing phase is the most critical and the clinicians have to pay attention to irrigation and pression to avoid pulp damages. Additional clinical studies have to be performed to confirm these results.
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Malnutrition and Perioperative Nutritional Support in Retroperitoneal Sarcoma Patients: Results from a Prospective Study.
Ann Surg Oncol2020 Jun;27(6):2025-2032. doi: 10.1245/s10434-019-08121-0.
Previtali Paola, Fiore Marco, Colombo Jacopo, Arendar Irina, Fumagalli Luca, Pizzocri Marta, Colombo Chiara, Rampello Nicolò N, Mariani Luigi, Gronchi Alessandro, Codazzi Daniela
Abstract
INTRODUCTION:
Retroperitoneal soft tissue sarcomas (RPSs) are mesenchymal neoplasms. The prevalence of protein energetic malnutrition (PEM) and its impact in RPS patients who were candidates for surgery is unknown.
MATERIALS AND METHODS:
A prospective feasibility study enrolled 35 patients with primary RPS who were candidates for extended multivisceral resection. PEM was screened at enrollment. Preoperative high protein ?-hydroxy-?-methyl butyrate oral nutritional support (ONS) was provided according to the degree of PEM. After surgery, nutritional support followed standard practice, targeting at least 1 g/kg/day protein and 20 kcal/kg/day caloric intake within the third postoperative day (POD). PEM was re-evaluated before surgery on POD 10, and at 4 and 12 months after surgery. Primary outcomes were the patient's compliance to preoperative ONS and the physician's compliance to postoperative nutritional targets.
RESULTS:
PEM was documented in 46% of patients at baseline; ONS met a 91% adherence (overall well tolerated). After ONS, PEM reduced to 38% (p?=?0.45). The postoperative caloric target was reached on day 4.1 (standard error?±?2.7), with a protocol adherence rate of 52%. On POD 10, 91% of patients experienced PEM, the worsening of which was greater after resection of four or more organs (p?=?0.06). At 4 and 12 months after surgery, almost all patients had fully recovered. A significant correlation between PEM at surgery and postoperative complications was found (p?=?0.04).
CONCLUSIONS:
Relevant PEM prevalence in RPS is documented for the first time. PEM correlates with greater morbidity. In this setting, preoperative ONS was feasible and well-tolerated. Disease-related factors for PEM and the ideal perioperative caloric target in the context of extended multivisceral resection need to be further investigated. Nutritional support should be included in enhanced recovery after surgery programs for RPS.
TRIAL REGISTRY:
ClinicalTrials.gov identifier: NCT03877588.
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Piezoelectric implant surgery and immediate loading in the aesthetic zone.
Minerva Stomatol2019 Oct;68(5):217-225. doi: 10.23736/S0026-4970.19.04281-X.
Baldi Domenico, Colombo Jacopo, Schierano Gianmario, Carossa Stefano, Rebaudi Alberto, Pesce Paolo, Pera Paolo
Abstract
BACKGROUND:
Replacement of missing teeth in the anterior aesthetic zone has challenged clinicians with fixed, removable or implant prosthodontics. Problems have been encountered could void aesthetic and functional results. The aim of this study was to present a clinical case report in which clinician challenged a very strong aesthetic case with an innovative surgical and prosthetic approach.
METHODS:
A patient who presented to the authors with a maxillary central incisor that had a hopeless prognosis secondary to acute localized severe periodontitis. The patient was treated by extracting the tooth and replacing it immediately with a platform switched implant using piezoelectric surgery. The implant was then restored with an immediate, non-occlusal loading provisional restoration.
RESULTS:
The implant became osseointegrated and was ultimately restored with a definitive abutment and crown restoration.
CONCLUSIONS:
Innovative approaches could represent a valid alternative to restore patients functionally and aesthetically.
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Temperature variations in pulp chamber: an in-vitro comparison between ultrasonic and rotating instruments in tooth preparation. Part 1.
Minerva Stomatol2020 Feb;69(1):14-20. doi: 10.23736/S0026-4970.19.04279-1.
Baldi Domenico, Colombo Jacopo, Robiony Massimo, Menini Maria, Bisagni Elisa, Pera Paolo
Abstract
BACKGROUND:
The purpose of this study was to analyze pulpal temperature increase generated by prosthodontic margin repositioning and finishing with ultrasonic and rotating instruments. The temperature changes recorded were also correlated with the residual dentin thickness.
METHODS:
A sample of 32 human extracted molars was selected. The teeth were endodontically treated and prepared with prosthetic round chamfer preparation. Then, they were inserted in plaster cubes up to the cement-enamel junction, leaving the apical portion pervious for inserting the thermocouple probe. The conventional technique, which involves the use of a high-speed contra-angle handpiece, was compared with an ultrasonic method (Crown Prep, Mectron, Carasco, Italy). For margin repositioning and finishing, two walls were randomly selected for each tooth: one was included in the test group and cut with the piezoelectric instrument (Multipiezo Touch TipHolder DB2, Mectron, Carasco, Genoa, Italy), the other one was inserted in the control group and cut with the high speed contra-angle handpiece (Kavo, Biberach, Germany). To standardize the operator-dependent parameters, it was used a mechanical arm controlled by a computer. These parameters were the pressure exerted on the dental wall, the cutting length and the time required for margin repositioning and finishing. For both test and control group, test phase consisted in a first stage of margin repositioning using an ultrasound tip or a diamond bur with a greater granulometry (120 µm for the ultrasound tip and 125 µm for the diamond bur), followed by a second finishing step conducted by an ultrasound tip or a diamond bur with smaller granulometry (60 µm for the ultrasound tip and 30 µm for the diamond bur). During these stages the intrapulpal temperature was recorded thanks to a thermocouple. Before and after these steps, the thickness of the remaining dental walls was measured with a caliber.
RESULTS:
The average pulpal temperature increase was 5.03±0.98 °C for the ultrasonic preparation (test group) and 3.55±0.95 °C for the conventional technique (control group). The difference was statistically significant (P value <0.001). However, neither of the instruments reached the critical level of 5.5 °C reported in the literature. The mean initial dentin thicknesses was 1.82±0.47 mm for the control group and 1.59±0.54 mm for the test group but the analysis of the residual dentin thicknesses revealed a greater reduction of the walls worked up with high speed contra-angle handpiece (mean 0.9±0.5 mm), which was therefore more aggressive than the ultrasonic instrument (mean 1.1±0.5 mm). A very weak negative correlation was present between the thickness of the wall at baseline and the increment of temperature.
CONCLUSIONS:
Within limitations of this study, temperature increasing of ultrasonic instruments shows a statistical difference related to rotary ones. But, as literature shows, the ultrasonic advantages are margin precision, preservation of soft tissues and reduction of operating times. Furthermore, in relation with results of this study, they could be considered safe for pulp vitality because the increase in pulpal temperature is similar to traditional instruments and it does not exceed the critical level of 5.5 °C.
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Unexpected postpneumonectomy exertion-induced acute right heart failure.
Tumori2018 Dec;104(6):NP61-NP67. doi: 10.1177/0300891618812682.
Colombo Jacopo, Arendar Iryna, Pastorino Ugo, Codazzi Daniela
Abstract
INTRODUCTION:
Thoracic surgery can have serious side effects. Accurate preoperative evaluation to assess which patients with anatomically resectable disease are suitable candidates for surgery is mandatory.
CASE DESCRIPTION:
A 58-year-old man, scheduled for left pneumonectomy for lung cancer, passed all the preoperative examinations suggested by guidelines and underwent the surgery. Three days after pneumonectomy, he experienced hemorrhagic shock and subsequent acute respiratory distress syndrome. Fifteen days after left pneumonectomy, he experienced exertional acute right heart failure (ARHF). We administered sildenafil and he improved, clinically and echocardiographically. During the following 2 years of follow-up, he was asymptomatic at rest but under exercise test, he developed pulmonary hypertension with echocardiographic signs of right ventricular impairment.
CONCLUSIONS:
In patients scheduled for major lung resections, preoperative stress echocardiography may give an adjunctive value to cardiopulmonary exercise test, quantifying the exercise-induced reduction in right ventricle ejection fraction and identifying asymptomatic patients at risk for postoperative ARHF.
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An unexpected blood thief: the subacute presentation of a spontaneous Ilio-iliac arterio-venous fistula.
Intensive Care Med2018 Nov;44(11):1953-1954. doi: 10.1007/s00134-018-5246-1.
Caranzano Leonardo, Colombo Jacopo, Cartolari Roberto, Perren Andreas
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Correlation between Insertion Torque and Implant Stability Quotient in Tapered Implants with Knife-Edge Thread Design.
Biomed Res Int2018 ;2018():7201093. doi: 10.1155/2018/7201093.
Baldi Domenico, Lombardi Teresa, Colombo Jacopo, Cervino Gabriele, Perinetti Giuseppe, Di Lenarda Roberto, Stacchi Claudio
Abstract
Aim:
To evaluate the correlation between insertion torque (IT) and implant stability quotient (ISQ) in tapered implants with knife-edge threads.
Methods:
Seventy-five identical implants (Anyridge, Megagen) were inserted by using a surgical drilling unit with torque control and an integrated resonance frequency analysis module (Implantmed, W&H). IT?(N/cm) and ISQ were recorded and implants were divided into three groups ( = 25) according to the IT: low (<30), medium (30 < IT < 50), and high torque (>50). ISQ difference among groups was assessed by Kruskal-Wallis test, followed by Bonferroni-corrected Mann-Whitney -test for pairwise comparisons. The strength of the association between IT and ISQ was assessed by Spearman Rho correlation coefficient (? = 0.05).
Results:
At the pairwise comparisons, a significant difference of ISQ values was demonstrated only between low torque and high torque groups. The strength of the association between IT and ISQ value was significant for both the entire sample and the medium torque group, while it was not significant in low and high torque groups.
Conclusions:
For the investigated implant, ISQ and IT showed a positive correlation up to values around 50?N/cm: higher torques subject the bone-implant system to unnecessary biological and mechanical stress without additional benefits in terms of implant stability. This trial is registered with NCT03222219.
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Sevoflurane improves respiratory mechanics and gas exchange in a case series of infants with severe bronchiolitis-induced acute respiratory distress syndrome.
Clin Case Rep2018 May;6(5):920-925. doi: 10.1002/ccr3.1490.
Nacoti Mirco, Colombo Jacopo, Fochi Oliviero, Bonacina Daniele, Fazzi Francesco, Bellani Giacomo, Bonanomi Ezio
Abstract
This report describes the successful use of a new intervention to improve respiratory mechanics and gas exchange in a relatively homogeneous group of infants with severe bronchiolitis-induced PARDS after failure of conventional treatment. These results may open a new interesting area of research and management for PARDS patients.
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Light in the dark: F-FDG PET/CT in Staphylococcus aureus bacteremia of unknown origin.
Intensive Care Med2018 04;44(4):488-489. doi: 10.1007/s00134-017-4994-7.
Colombo Jacopo, Elzi Luigia, Treglia Giorgio, Perren Andreas
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Pharmacokinetic/Pharmacodynamic Target Attainment During Extracorporeal Membrane Oxygenation: Does the Circuit Matter?
Pediatr Crit Care Med2017 11;18(11):1090-1091. doi: 10.1097/PCC.0000000000001307.
Colombo Jacopo, Scaffidi Ilenia
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Evaluation of a New Ultrasonic Insert for Prosthodontic Preparation.
Int J Prosthodont;30(5):496-498. doi: 10.11607/ijp.5378.
Baldi Domenico, Menini Maria, Colombo Jacopo, Lertora Enrico, Pera Paolo
Abstract
PURPOSE:
This paper describes a new ultrasonic instrument (tipholder DB1 with crown prep tip inserts) designed to optimize prosthodontic margin repositioning and finishing.
MATERIALS AND METHODS:
The insert movement was assessed, and it was demonstrated that tipholder DB1 provides its inserts with an elliptical-like movement, making the entire insert surface able to cut. Then, 20 extracted teeth were prepared using tipholder DB1, sonic instruments, and traditional drills. Dental surface roughness produced using each of the three tools was measured using a roughness tester. Results were compared using univariate analysis of variance and Bonferroni post hoc test.
RESULTS:
The roughness produced using tipholder DB1 with crown prep insert presented no statistically significant differences compared to the roughness produced using sonic instruments and traditional drills.
CONCLUSION:
Tipholder DB1 with crown prep inserts is a promising treatment for margin repositioning and finishing.
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A Commentary on Albumin in Acidosis.
Int J Crit Illn Inj Sci -
Acetazolamide in Metabolic Alkalosis: Cosmetic or Therapy?
Pediatr Crit Care Med2017 02;18(2):201. doi: 10.1097/PCC.0000000000001039.
Colombo Jacopo, Codazzi Daniela
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Predictors of Outcomes After Pediatric Cardiac Surgery: A Proposal to Improve the Vasoactive-Ventilation-Renal Score.
Ann Thorac Surg -
Nutritional Status Assessment in Critically Ill Children.
Crit Care Med2016 10;44(10):e1007. doi: 10.1097/CCM.0000000000001894.
Colombo Jacopo, Pellicioli Isabella, Bonanomi Ezio
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Ventilation-associated pneumonia and probiotics: many clues do not make evidence.
Intensive Care Med2016 Sep;42(9):1511-2. doi: 10.1007/s00134-016-4366-8.
Colombo Jacopo, Codazzi Daniela, Langer Martin
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Multiplex Tests in Critically Ill Children With Severe Lower Respiratory Tract Infections.
Pediatr Crit Care Med2016 06;17(6):586-7. doi: 10.1097/PCC.0000000000000749.
Colombo Jacopo, Bonanomi Ezio
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Lung Transplantation Is Much More Than Lungs and Fluids.
Anesth Analg2016 09;123(3):794-5. doi: 10.1213/ANE.0000000000001375.
Colombo Jacopo, Arena Angela, Codazzi Daniela
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You Are Hypoalbuminemic and So You Are Sick?but What Can I Do for You? Your Amino Acid Gram Could Help Me.
Pediatr Crit Care Med2016 05;17(5):479. doi: 10.1097/PCC.0000000000000696.
Colombo Jacopo, Codazzi Daniela
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Optimise nutrition before surgery: A family-centred intervention may be the solution.
Aust Crit Care -
Capnometry in children with bronchiolitis: A pathophysiological point of view.
Respir Med2016 08;117():280-1. doi: 10.1016/j.rmed.2016.03.002.
Colombo Jacopo, Bronco Alfio, Lippolis Valeria, Bonanomi Ezio
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Difficult to wean patients.
Aust Crit Care -
What is new in critical illness and injury science? Acetazolamide in decompensated respiratory failure!
Int J Crit Illn Inj Sci -
Intra-abdominal candidiasis and probiotics: we know little but it's time to try.
Intensive Care Med2014 Feb;40(2):297-8. doi: 10.1007/s00134-013-3172-9.
Colombo Jacopo, Arena Angela, Codazzi Daniela, Langer Martin
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?-adrenergic agonist infusion during extracorporeal lung perfusion: effects on glucose concentration in the perfusion fluid and on lung function.
J Heart Lung Transplant2012 May;31(5):524-30. doi: 10.1016/j.healun.2012.02.001.
Valenza Franco, Rosso Lorenzo, Coppola Silvia, Froio Sara, Colombo Jacopo, Dossi Roberto, Fumagalli Jacopo, Salice Valentina, Pizzocri Marta, Conte Grazia, Gatti Stefano, Santambrogio Luigi, Gattinoni Luciano
Abstract
BACKGROUND:
We recently showed in a pig model of ex vivo lung perfusion (EVLP) that lung edema correlates with glucose consumption. We investigated whether salbutamol, a ?-adrenergic receptor agonist known to upregulate fluid transport in the lung, modulates glucose concentration in the perfusate during EVLP.
METHODS:
Lungs from domestic pigs underwent normothermic EVLP. At the end of controlled reperfusion, lungs were ventilated and perfused for 60 minutes, then randomized to salbutamol (?-Agonist) infusion or placebo (Control) for 180 minutes. Functional parameters were assessed.
RESULTS:
In the ?-Agonist group, glucose concentration decreased over time more than corresponding Control values (analysis of variance [ANOVA], p = 0.05). Mean pulmonary artery pressure (mPAP) was 16 ± 1 mm Hg in the ?-Agonist group vs 21 ± 1 mm Hg in the Controls (ANOVA p < 0.05). Baseline mPAP was correlated with the drop of mPAP after the ?-agonist infusion (R(2) = 0.856, p < 0.05). Dynamic compliance dropped from 51 ± 10 to 31 ± 6 ml/cm H(2)O in the ?-Agonist group and from 60 ± 4 to 21 ± 3 ml/cm H(2)O in the Control group (ANOVA, p < 0.05 ?-agonist vs Control). The ? partial pressure of oxygen/fraction of inspired oxygen was 418 ± 15 and 393 ± 12 mm Hg in the ?-Agonist and Control groups, respectively (t-test p = 0.106).
CONCLUSIONS:
Glucose concentration in the perfusate was affected by salbutamol. Salbutamol was associated with lower pulmonary pressures and better lung mechanics. These data suggest a possible role for salbutamol as a pharmacologic adjunct during EVLP before transplantation.
Copyright © 2012 International Society for Heart and Lung Transplantation. Published by Elsevier Inc. All rights reserved.
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